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Design of an optofluidic platform to study nutrient response in cell communities

June 22 @ 3:00 pm - 4:00 pm

Date – 22 nd June, 2017 (Thursday)

Time – 3.00 PM

Venue – ESB 244

Speaker – Rudra Vaswata Roy Choudhury (EE14S047)

Guide – Dr. Anil Prabhakar

 GTC Members

Dr. Enakshi Bhattacharya (Chairperson)

Dr. Shanti Bhattacharya (M)

Dr. Ashish Kumar Sen (ME)

Abstract

An important aspect of cell biology is to study the effect of micro-environmental conditions on cell growth. Investigation of the cellular response to different nutrient conditions can provide answers to important cellular processes like cell division, differentiation, etc. This is usually achieved by culturing cells in macro bioreactors, followed by either studying cell growth through the measurement of optical density (OD) or imaging cells over a period of time using conventional microscopes to look at morphological characteristics of the cell. However, the current methods are complex, costly and hence their application is limited.

The presented work proposes an optofluidic platform to study nutrient responses in cell communities using portable microscopy. A microfluidic cell culture device has been designed which generates nutrient gradients across multiple chambers where colonies of cells are grown and maintained. In addition to this, a cellphone based brightfield microscope is designed that can image biological samples and micro-organisms at a suitable magnification and resolution. The microfluidic device is augmented to the cellphone microscope which leads to the development of an integrated lab-on-chip platform for live cell imaging.

All are cordially invited.

Details

Date:
June 22
Time:
3:00 pm - 4:00 pm
Event Category:

Venue

ESB 244
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